ELISA inibition technique for the demonstration of sulphones in body fluids. Comparison of two ELISA methods

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ELISA inhibition technique for the demonstration of sulphones in body fluids

Sum mary A n enzyme-linked immunosorbent assay ( ELISA) for sulphones in urine is described . This method for monitoring dapsone ( D D S) self­ administration is comp ared with the quantitative d etermination of DDS/ creatinine (D/C) rat io s, currently used . Urine samples collected from 1 0 volunteers o n 1 4 consecutive days after taking single doses of 1 00 mg D D S are studied , together w...

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ELISA inhibition technique for the demonstration of sulphones in body fluids. I. Sulphones specific antibody-enzyme conjugate.

Two enzyme-linked immunosorbent assays ( ELISA) for sulphones in body fluids were adapted to measure sulphones in blood dried on filter paper. The more sensitive modification , based on competition between dapsone (DDS) and enzyme-dapsone conjugate , detects sulphone in blood extracts up till 6 days following 1 00 mg DDS intake . Application to monitor patient compliance is demonstrated, using ...

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ژورنال

عنوان ژورنال: Leprosy Review

سال: 1981

ISSN: 0305-7518

DOI: 10.5935/0305-7518.19810031